UltiMate 3000 RSLCnano – Standard Applications
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Figure 20: Typical chromatogram for Pre-concentration of 0.5 pmol Cyt C onto a monolithic column
Tip: When the trap column is switched in line with the analytical column, a large
positive peak is detected at 214 nm. This is due to the different UV absorbance of
the ion-pairing agents used in the loading and analytical solvent.
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