UltiMate 3000 RSLCnano – Standard Applications
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Property Setting
Sample Protein mix digest, prepared according to the included instruction sheet
Injection volume Sample: 10 μL
Fractions: 20 μL
Gradient RP basic 4-60% B in 15 min,
90% B for 5 min,
25 min equilibration
UV detection 214 nm
Fractions Every minute for 20 minutes
Loading time 7 min (may vary with different injection volume/routine)
Gradient RP acidic Isocratic 4% for 10min
4% to 55% B in 30 min
90% B for 5 min
15 min equilibration
WPS temperature
5°C
Flow rate cap 6 µL/min
Loading flow 5 μL/min
Flow rate nano 300 nL/min
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