Function and setup Biometra TRIO
16 Version 03_19
The Biometra TRIO Thermal Cycler features an automatic restart. If a power failure
occurs during the run the instrument will continue the run when the power returns. In
case of long- term power failure (longer than 30 minutes) the instrument keeps the
sample block at 4°C(freeze step) and the user can decide to repeat the run with the
same samples or to discard them.
This instruction manual provides information on how to use all models of the Biometra
TRIO Thermal Cycler most effectively.
3.3 Special features
3.3.1 Thermal Block
The Biometra TRIO Thermal Cycler is available with different block formats. The 48
well version can be used with standard 0.2 ml tubes, strips or plates. With 30 well
blocks the Biometra TRIO can be used with 0.5 ml tubes and the combi block version
offers the flexibility to incubate 0.2 ml tubes, strips or plates or 0.5 ml tubes. With a
sample capacity of up to 144 samples in parallel the Biometra TRIO 48 can also be
used for high throughput applications.
By the rubber sealing at the lid an encapsulated space is formed when the lid is closed.
The closed room serves to improve the temperature uniformity of the sample block
and avoids formation of condensed water at the final PCR cooling step. Furthermore
all blocks are perfectly sealed to prevent condensed water from penetrating the Peltier
elements underneath the sample block and other parts of the electronics. By the per-
fect sealing the Peltier elements are protected and the instruments life time is ex-
panded.
3.3.2 High Performance Smart Lid
The Biometra TRIO heated lid has been optimized to heat twice as fast as with older
models and thus helps to shorten protocol run times. Moreover the heated lid serves
for two additional essential functions: It prevents the formation of condensed water at
the reaction tube portion located above the block surface level and it ensures reliable
contact between samples and thermal block by applying constant pressure. By the
integrated clutch mechanism the pressure applied to the reaction tubes is always same
regardless of the plastic ware height. The combination of the heated lid shape and the
reliable contact pressure ensure an even temperature distribution between samples
thus significantly improves the temperature uniformity.
With one press on the front button, the lid gently swings open and arrests in its end
position. By this mechanism the lid cannot fall down and the heated lid is out of the
radius of action when the user is inserting tubes or plates. Thus the risk of injury by
violent pressure or the risk of burn by hot surfaces is prevented.
3.3.3 Display
The Biometra TRIO thermal cycler features a 7" state-of-the-art color touchscreen user
interface. The touchscreen is built-in at a flat angle to ensure reflection-free viewing
and ergonomic programming. For programming PCR protocols the software incorpo-
rates Biometra's proven spreadsheet philosophy and alternatively offers graphical
programming. One touch of a button leads from the spreadsheet to the alternative
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