Biometra TRIO Adaptation of programs
Version 03_19 95
12 Adaptation of programs
If programs are exchanged between different thermal cycler models or of the sample
block is exchanged programs might need to be adapted prior to start or during editing.
The following table provides an overview for possible adaptations:
Maximum gradient range
is exceeded.
A program for the 96 well gradient
silver block with a programmed
temperature range > 30°C is to be
started on a 96 well gradient alumi-
num block and therefore exceeds
the block's maximum gradient
range.
The temperature range of the gradient will be re-
duced based on the average temperature of the max-
imum permissible value. If the average temperature
of the programmed gradient is, for example, 60°C
and the temperature range is ± 20°C, it will be re-
duced to 60°C ± 15°C.
A program including a
gradient step is trans-
ferred to a non- gradient
instrument.
The program is including a gradient
step started on a block without gra-
dient function.
The gradient will be deleted and the average tem-
perature/annealing temperature will be used for this
step. If the average temperature/annealing tempera-
ture of the programmed gradient is for example 60°C,
this value will be used for the step.
A program for the 96 well silver
block with a programmed gradient
temperature below 20°C is to be
started on a 96 well aluminum block
and falls below minimum permitted
temperature.
The lower temperature is increased to the minimum
permitted value. If for example a gradient is pro-
grammed from 5°C to 25°C the gradient is changed to
20°C to 25°C.
A program including a
temperature optimization
step (TOS) is transferred
to an instrument that
does not offer this func-
tion
A TOS program is transferred from
the Biometra TRIO to another ther-
mal cycler
The mean value of the programmed temperatures in
the TOS step for the left and right sample block is
used.
The ramping rate exceeds
the limit
A program for the 96 well gradient
silver block is started on a 96 well
aluminum block.
The ramp rate will be adjusted to the maximum pos-
sible ramp rate for the installed block type. If the
programmed ramp rate is for example 8°C/s it will be
reduced to 6°C/s.
The ramping rate is lower
than the maximum
A program for the 96 well alumi-
num block is started on a 96 well
silver block.
The ramp rate will be adjusted to the maximum pos-
sible ramp rate for the installed block type. If the
programmed ramp rate is for example 6°C/s it will be
increased to 8°C/s.
User defined ramping
rate
The ramping rate for a program
step is lower than maximum value
The user defined value is maintained and does not
become adjusted
Note: If programs need to be adapted to the installed sample block prior to the start or
editing a message will be displayed. The user can confirm or can deny the adaptations.
If during program start the necessary adaptation is denied the program cannot be
started by the instrument.
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