Creating, editing and saving programs Biometra TRIO
44 Version 03_19
7.6 Edit parameter or step
The software offers two different options to edit program steps or single parameters:
1. In the programming spreadsheet or the graphical view single parameters can be
activated and values entered.
2. To edit all parameters in a program step the software offers the edit step screen
(see Figure 22). Furthermore, in this screen it can be switched between steps and
parameters entered for multiple steps (Multi Step Programming).
Activate a parameter in the tabular or graphical view (see chapter "Programming
modes") and enter the desired values or select a program step and press the [EDIT STEP]
button.
The following parameters can be edited:
Temperature in °C for a step. The target temperature can be set between
3.0°C and 99.0°C in tenths of a degree.
Hold time for a step in hh:mm:ss. Between 0 and 8 hours, 0 and 59
minutes and 0 to 59 seconds can be set.
To program a pause and set the holding time indefinitely, enter ∞ in one
of the input boxes for hh:mm:ss.
Defines the step number the program jumps back to.
Defines the number of repeats. The maximum number of repeats is 999.
Temperature increment to increase or decrease the set temperature
with each cycle by the defined value.
The increment can be set to ± 20°C.
Time increment (e.g. for long range PCR). Increases the set time with
each cycle by the defined increment. The increment can be set from 0s
to 240s.
Average ramp rate between steps. The average ramp rate can be set
from 0.1°C/s to max in tenths of a degree (The maximum ramp rate is
sample block specific). The ramp rate specifies the speed at which the
selected step is reached with.
Temperature Optimisation Step (TOS). For optimisation of new PCR pro-
tocols very often gradient enabled Thermocyclers are used. The Biomet-
ra TRIO offers no gradient function but by the temperature optimisation
step (TOS) function pograms making use of the three blocks and deliver-
ing different annealing temperatures at the defined step can be created.
It is possible that in some models this display may show a slightly higher
maximum ramping rate than is achieved according to the technical spec-
ifications of the device. This is technically justified and achieves opti-
mum control of the maximum value. In fact, the maximum heating and
cooling rate is achieved as specified in the technical specifications.
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