Fluid Imaging Technologies FlowCAM - Page 121

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VisualSpreadsheet can determine the total volume it imaged with the following calculation:
Total Volume Imaged = Number of Images Taken during the Run × Volume per Picture (µm
3
)
VisualSpreadsheet can calculate PPM using either the Volume (ESD) or Volume (ABD) particle volume
measurements.
Total Volume of Particles using ESD or ABD (µm
3
)
PPM = ————————————————————— X 10
6
Total Volume Imaged (µm
3
)
Particle Count 1 x 10
12
(µm
3
)
P/mL = ———————————— X ———————
Total Volume Imaged (µm
3
) 1 (mL)
To locate the values used in the above calculations, from the Main window:
· For Height and Width, access Setup > Context > Capture (tab) > Acceptable Region (section) to
view the total number of pixels from left to right and from top to bottom.
· For Depth, access Setup > Context > Flow Cell (tab) > Flow cell (section) for the “Flow cell
depth” value (populated from the Flow Cell Database file).
· For total number of camera images, access Setup > Context > Run Summary (tab) > Images >
Total.