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¨ Fluidics
¨ Filter
¨ PMT/Scatter (if required)
¨ Stop
¨ Load (if desired)
· Click OK in the Context Window
§ Test the settings before you do an analysis by starting AutoImage Mode (No Save) or Trigger
Mode (No Save).
§ Main Window: Setup > AutoImage Mode (No Save) or Trigger Mode (No Save). Note: If
working with a peristaltic style pump, turn on the pump before you click on the No Save
mode.
· Determine if the FlowCAM is identifying particles of interest to you and segmenting
them correctly.
· Close the No Save run and make changes in the Context Settings and repeat as needed
§ Insure that an appropriate amount of sample is in the sample introduction funnel/pipette
§ Main Window: Analyze > Mode of your choice
· Follow subsequent prompts
· In the window where the data set is named please note that when you click “Save” the
run will begin
§ Allow data collection to end based on the preset stop conditions or terminate manually if
desired/required.
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