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Fluid Imaging Technologies FlowCAM - Page 63

Fluid Imaging Technologies FlowCAM
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Context… Filter
Basic Size Acquisition Filter - This function is the primary image filtration step that occurs during the
processing of the raw camera image. The user can choose to filter using ESD (Equivalent Spherical
Diameter) or ABD (Area Based Diameter) or can choose No Size Filter (these terms are defined in more
detail below). The user sets the minimum and maximum Diameter values (µm) of this image filter in
order to specify the size range of the particles of interest that will be saved. The software always
segments out each particle as defined by the Threshold parameters but it only saves images of particles
that fall within this filter’s size range. For example, a raw camera image may contain hundreds of
particles with ESD values that range from 1-90 µm but the user is only interested in capturing/imaging
particles with an ESD value of 10-20 µm. Use ESD should be selected with Diameter Min set to 10 and
the Diameter Max set to 20. Then as VisualSpreadsheet analyzes the raw image and attempts to
capture/image particles, it will ignore (and not capture) any of the particles that fall outside of the
specified 10-20 µm range. Recommended minimum Diameter ESD sizes are as follows:
Objective
Min. Size
(Diameter ESD)
2x 20 µm
4x 12 µm
10x 4 µm
20x 2 µm
ESD (Equivalent Spherical Diameter) - The diameter calculated as the mean feret measurement based
on 36 sample measurements (conducted every 5°).

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