Connect a column
CAUTION
Before use, check that the column is not damaged or otherwise
defective. Damaged or defective columns may leak.
Follow the instructions below to connect a column to the system (connectors should be
treated aseptically when connecting):
ActionStep
Remove the welded tubing end
from the Bottom Inlet tubing on
the column (keep the tubing
ends for when the column shall
be sealed).
1
If necessary, fill the inlet tubing
with 20% ethanol or 2% benzyl
alcohol, using e.g. a squirt bottle.
2
Remove the protective end cap
from the column inlet tubing on
the flow kit (1 in figures) and,
using a TC clamp, connect it to
the Bottom Inlet tubing on the
column.
3
Repeat the steps above for the
Column outlet connector (2 in
figures):
Connect this tubing to the Top
Inlet tubing connector on the
column.
4
132 ÄKTA ready Operating Instructions 28960345 AD
9 Column installation
9.2 Installing a column
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