Gilson 333 - Page 90

90
FRONT PANEL CONTROL | 333/334 HPLC PUMPS
FRONT PANEL CONTROL
Seal Test
From the Start Screen press Edit > GLP > Seal.
This procedure is designed for installing new piston seals with isopropanol. For a different
solvent, adapt the flow rate in proportion to the viscosity of the solvent used. The seal installation
procedure is divided into two parts:
Priming for two minutes at a fixed flow rate to the drain. See the table below.
Pumping at a preprogrammed gradient (ascending, then descending flow rate) against
the back pressure provided by the supplied capillary tubing, for 14 minutes. If the pressure
indicated in the table is reached before 2 minutes, then the software interrupts the positive
flow rate gradient, and maintains, during 10 minutes, the flow rate at the value that generated
this pressure, otherwise it goes to the flow rate indicated in the table and maintains it during 10
minutes. At the end of this time, the flow rate linearly decreases to zero in 2 minutes.
Pump Head Prime GLP/Seal GLP/Leak
Flow Rate
Fixed
mL/min
Purge Flow
Rate Fixed
mL/min
Procedure
Flow Rate
Default
mL/min
Procedure
Pressure
Default
MPa
Procedure
Pressure
Default
MPa
Procedure
Flow Rate
Default
mL/min
33X-H3 50 50 15 15 15 0.5
33X-H2 35 35 25 45 45 0.5
30X-5 5 3.75 5 45 45 -
30X-10 10 7. 5 10 45 45 -
30X-25 25 18.75 12 21 28 -
30X-50 50 22.5 12 10.5 14 -
30X-100 100 22.5 12 5.2 7 -
30X-200 200 22.5 12 2.6 3.5 -