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FRONT PANEL CONTROL | 333/334 HPLC PUMPS
FRONT PANEL CONTROL
Manual Operations
Hydraulic Priming
From the Start screen press Manual > Prime to reach the Prime Screen.
Priming is an essential precursor to operating any pump. Any actively associated pump (333,
334, or 30X) can be primed from the 333 Pump (master).
Running a pump head dry, even for a short time, will damage the instrument.
Ensure the PPMM is fitted with drain tubing before priming the pump.
1. Fill the solvent bottle(s) with degassed, high pressure liquid chromatography (HPLC) grade
solvent(s) and immerse the filter connected to the inlet tubing for each pump into the solvent.
2. Open the purge valve on the PPMM.
3. Press the Start soft key to prime the corresponding channel. Self-priming is generally
achieved after two minutes. If self priming is achieved, skip to step 8. If not, press Stop, and
then perform steps 4–7.
4. Disconnect the drain tubing from the purge valve, then replace it with a priming syringe
(part number 36460058) equipped with its adapter.
5. Press Start, then draw solvent into the syringe while continuing to pump. When solvent
appears in the syringe, press Stop.
6. Reconnect the tubing and continue priming for as long as is necessary. No bubbles should
be present in the solvent flowing through the tubing. Press Stop to stop pumping.
7. Repeat the procedure for other pumps in the system as needed.
8. Press ESC to return to previous screen. All pumps must be stopped before you can leave
the Prime screen.
While priming, the low pressure limit is inactive. To protect the column, the high pressure limit
is automatically set to 30 bar (435 psi). If the purge valve was not opened, the screen displays
the following message: Open Purge Valve.
9. After priming, use the GLP function Seal to initialize the piston seals (if new). See
Seal Test Procedure on page 91.
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