10. Operating Cryogenic Trap
10.1 Configuring Cryotrap
The cryotrap can be used with any of the OPTIC-4 standard method types. Prior to that, it should be
enabled in the system configuration. This can be done through the Evolution Workstation software.
Ensure that the OPTIC-4 control unit is switched on and communicating with the Evolution
Workstation software on the PC. Select Configuration - System Configuration - General. Check the
cryotrap option. Click on OK to confirm the configuration.
10.2 Cryotrap Temperature Profile
When the cryotrap is enabled, the trap temperature profile will be added to any method you create.
An example is shown in Chapter 9 “Advanced OPTIC-4 features”.
The following parameters can be set in the profile:
Cryo Low Temperature:
This is the initial temperature, or cryo-focusing temperature, to cold-trap the analytes of interest.
Any temperature between –150 C and +350 C may be set but this temperature will normally be in
the range –150 C to 0 C.
Cryo Hold Time:
This is the time required after receiving a run signal for the sample to be fully transferred from the
inlet to the cryotrap.
NOTE:
If sample is introduced via a splitless injection system, “Cryo Hold Time” should be a bit
longer than “Transfer Time”. The available time setting range is 00.00 to 120:00
minutes, but it is convenient to set “Cryo Hold Time” in the range 1:00 to 5:00 minutes.`
CAUTION!
It is strongly advised not to hold the trap at subambient temperature for longer than 20
minutes. The ice is formed on the points of column near the trap ends if trap is kept
cold for a long time. This penomenon can cause analytical problems and can also bring
damage to the column coating!
Cryo Heat Ramp Rate:
This is temperature ramp rate in degrees Celsius per second (C/sec) at which the trap heats from
the Cryo Low Temperature to the Cryo High Temperature. Currently the range available is 1 to
60C/sec
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