20
1 Installing GC Columns
To prepare a capillary column for installation
To prepare a capillary column for installation
Materials needed:
Capillary column
Column cutter (5181-8836)
Ferrules
0.27-mm id, for 0.10-mm id columns (5062-3518)
0.37-mm id, for 0.20-mm id columns (5062-3516)
0.40-mm id, for 0.25-mm id columns (5181-3323)
0.47-mm id, for 0.32-mm id columns (5062-3514)
0.74-mm id, for 0.53-mm id columns (5062-3512)
Gloves, clean
large (8650-0030)
small (8650-0029)
Inlet column nut (5181-8830)
Magnifying glass
Septum (may be old, used inlet septum)
1 Slide a septum, column nut, and conditioned ferrule onto the free end of
the column.
The tapered end of the ferrule should point away from the column nut.
2 Use the column cutter to score the column 2 cm from the end.
3 Break off the end of the column.
Hold the column against the column cutter with your thumb. Break the column
against edge of the column cutter.
4 Inspect the end for jagged edges or burrs.
If the break is not clean and even, repeat steps 2 and 3.
5 Wipe the outside of the free end of the column with a lint-free cloth
moistened with methanol.
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