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3 Installation
883 Basic IC plus
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53
Connecting the separation column
1
Removing the stoppers
￭ Remove the stoppers from the separation column.
2
Installing the inlet of the separation column
CAUTION
When inserting the column, ensure that it is inserted correctly
based on the marked flow direction.
There are 3 possibilities:
￭ Attach the column inlet directly onto the guard column or,
￭ if the guard column is connected to the separation column using
a connection capillary: Connect the column inlet to the guard col-
umn outlet capillary using a PEEK pressure screw (6.2744.070) or,
￭ if no guard column is used (not recommended): Connect the col-
umn inlet capillary to the inlet of the separation column using a
short pressure screw (6.2744.070).
3
Rinsing the separation column
￭ Place a beaker under the outlet of the separation column.
￭ Start manual control in MagIC Net and select the high-pressure
pump: Manual ▶ Manual control ▶ Pump
– Flow: Increase gradually up to the flow rate recommended
in the column leaflet.
– On
￭ Rinse the separation column with eluent for approx. 10 minutes.
￭ Stop the high-pressure pump in the manual control in MagIC Net
again: Off.
4
Removing the coupling
￭ Remove the coupling (6.2744.040) from the column outlet capil-
lary.
5
Installing the outlet of the separation column
￭ Fasten the column outlet capillary to the column outlet using a
short PEEK pressure screw (6.2744.070).
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