Using the Calibration Programs
9-8
Figure 9-4 How to dispense diluent
4. When the dispensing is finished, press [ENTER] to close the box;
5. Add 20µL of calibrator to the diluent and shake the tube to mix the sample.
z Be sure to keep dust from the prepared diluent.
z After mixing the calibrator with the diluent, be sure to wait 3 minutes before
running it.
z Be sure to run the prediluted calibrators within 30 minutes after the mixing.
z Mix any pre-diluted calibrator that has been prepared for a while before
running it.
z Be sure to evaluate predilute stability based on your laboratory’s sample
population and sample collection techniques or methods.
6. Present the mixed calibrator to the sample probe so that the tip is well into the tube, and
press the aspirate key. The System Status area will display “Running” and the analyzer
will start aspirating sample;
7. When you hear the beep and the sample probe is out of the tube, remove the calibrator.
The sample probe will retract into the analyzer and the analysis progress will be
displayed on the screen;
8. When the analysis is finished, the result will be displayed on the screen and the sample
probe will be replaced.
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