Operator’s Manual – OPTI CCA-TS2 9-5
9 OPERATING PRINCIPLES
Erythrocyte aggregation and sedimentaton may occur very quickly in syringes containing pathologic
blood samples and may adversely affect the measurement of ctHb in any analyzer. To prevent such errors,
rst insert the OPTI CCA-TS2 cassette into the analyzer to initiate calibration. Next, mix the syringe
sample well by rolling the syringe for at least 60 seconds, after expelling any trapped bubbles, then
immediately measure in the OPTI CCA-TS2.
The OPTI CCA-TS2 system aspirates blood in the same manner from syringes, capillaries or
ComfortSampler.
No changes are made to the aspiration rate, volume or timing. Therefore, there are no biases or
imprecision dependent upon the sample introduction method. Sufcient volume must, however, be
present in syringes (0.25 mL in a 1 mL syringe) to prevent mechanical interference between the syringe
plunger and the syringe adapter.
Errors in blood analysis on properly collected samples may result from improper mixing of the sample
after collection and before measurement; contamination with room air resulting from failure to expel any
trapped bubbles after collection; and from metabolic changes in the sample.
Serum samples should be obtained by collecting blood in an untreated blood collecting tube. The sample
should stand for 30 minutes to allow the clot to form prior to centrifugation. After centrifugation, remove
the serum from the clot, and cap or seal the sample tube. If storage is required, the sample should be
tightly capped, refrigerated at 4 to 8 °C for no longer than 48 hours, and allowed to return to room
temperature, 15 to 30 °C, prior to analysis. Each laboratory should determine the acceptability of its own
blood collection syringes, capillaries and tubes and the serum or plasma separation products.
Variations in these products exist between manufacturers, and at times, from lot to lot.
NOTE: Serumisanunsuitablesamplematerialforaccurateglucoseanalysis,becausetheretention
timeoftheerythrocytesinthesampleistoolong.Theprocessofglycolysismayleadto
decreasedglucosevaluesinserumsamples.
9.5 Procedure
9.5.1 Materials Needed
Description Part Number
Sensor Cassettes see Chapter 10, Supplies
in various analyte congurations
Multi-Level Standard Reference Cassette BP7652
Calibration Gas Bottle BP7162
Hb Calibrator Cassette BP7653
Printer Paper HP0070
The OPTI CCA-TS2 automatically processes the sample through the necessary steps, then displays and
prints the results. For details of this operation, please refer to Chapter 5 of the Operator’s Manual.
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