Cl-2 Operator’s Manual – OPTI CCA-TS2
CHLORIDE ANALYTES
Interferences
The OPTI CCA Cl
-
sensor does exhibit a signiîš¿cant (greater than 2:1) positive interference from bromide,
iodide, interlipid and nitrite. Minor interference is observed from phenylacetic acid salicyate and
thiocynate. This testing was done on the OPTI CCA platform, with a standard OPTI style Chloride sensor.
No changes were made to the sensor or the measurement principle, so the interference of the OPTI CCA-
TS2 is equivalent to the OPTI CCA.
Heparin salts are the only acceptable anticoagulants. Other anticoagulants such as citrate, EDTA, oxalate,
and uoride cause signicant interferences to the electrolyte sensors.
Only clear, uncolored quality control materials, such as OPTI CHECK or OPTI CHECK PLUS brand
aqueous controls should be used with the OPTI CCA-TS2 system. Colored materials, including
proîš¿ciency testing materials, may interfere with the chloride measurement, or fail to be properly
aspirated
.
Reproducibility
Controls
Within run precision (S
wr
) and Total Precision (S
T
), were determined from 2 runs per day with 2 replicates
per run over a period of 10 days following the CLSI guideline EP5-A2. Typical results for 3 different
control levels are shown below.
Cl
-
(mmol/L) OPTI Check Level 1 OPTI Check Level 2 OPTI Check Level 3
Days Run 10 10 10
Total Average 95.3 107.1 115.6
Within Run St. Dev. (S
wr
) 0.6 1.4 0.5
Within Run % CV 0.7% 1.3% 0.4%
Total Precision St. Dev. (S
T
) 0.7 1.4 0.6
Total % CV 0.8% 1.3% 0.5%
Whole Blood
Within-run precision in whole blood samples was evaluated at 3 different concentrations on the OPTI
CCA-TS2. The table below shows the Averages, Standard Deviations and % CV calculated from 10
samples for each level.
Cl
-
(mmol/L) Whole Blood
Level 1 Level 2 Level 3
Average 104.1 141.3 57.5
St. Dev 0.3 1.0 0.3
%CV 0.28% 0.73% 0.51%
n 10 10 10
To Next Page
Loading...
Need help?
General