1 Introduction
1.1 About this Operating Manual
This Operating Manual will help you to operate your CyFlow™ Cube device. Emphasis is
placed on the installation, start-up and safe operation of the device.
The basic principles of flow cytometry can be found in the scientific literature (e.g. Howard
M. Shapiro, Practical Flow Cytometry, Wiley 2003).
This Operating Manual comes with the device. Read this Operating Manual thoroughly
before using the device. Please keep this Operating Manual for future reference.
This Operating Manual is divided in two parts. The first part, chapter 1 to 10, gives the
user all necessary information for safe handling, scope of delivery, to set up the device
and how to operate the device properly. The general use of the software and the typical
workflows are described in chapter 6, this chapter also is suitable to get to know the
functioning of your device.
In the second part, chapter 11, you will find a description of all software functions and a
detailed description of the user interface. This chapter is for reference.
There are product data sheets and assay manuals for the reagent kits and general
application notes available. Please also refer to these documents.
If you have any questions about the content of this manual or the use of the device,
contact your local Sysmex representative.
1.2 Typographical conventions
1.2.1 Safety information
Safety information and warnings are marked by symbols and signal words. Hazards are
categorised into four levels. All warnings are structured according to the same model. The
type of hazard is stated after the signal word. Then the consequences that may result
from the hazardous situation are described. And finally, how the hazardous situation can
be avoided. If applicable a hazard symbol is portrayed, to indicate the kind of hazard.
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