Section I – CD AutoPlus Maintenance
CD14 Tester & CD AutoPlus Autosampler User Guide—75-108-800 Rev. B—31 May 2021 107 of 130
EAR99 technology subject to restrictions on cover page.
7. Move the probe back to the caffeine solution and rinse
by a volume 1 mL greater than the previous rinse
volume.
8. Obtain a sample for UV analysis using the CD
AutoPlus controls.
9. Repeat steps 6 through 8 until a total of 10 samples of
the absorbing solution are obtained.
10. Run the samples on a UV/Vis spectrophotometer
starting with the two blanks and working from the
lowest rinse volume to highest.
11. Clean probes using DI water.
12. Use the following equation in order to determine the
rinse volume.
𝐴
𝐴
%
𝐴
%
∗ 99%
100%
𝐴
%
A
r
= the minimum absorbance required
A
100%
= the average absorbance of the 9th and 10th
readings of the absorbing solution
A
0%
= the average of the absorbance of the two blank
diluent solution readings
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