Section I – CD AutoPlus Maintenance
CD14 Tester & CD AutoPlus Autosampler User Guide—75-108-800 Rev. B—31 May 2021 106 of 130
EAR99 technology subject to restrictions on cover page.
Rinse Volume
Materials required
1. One UV/Vis spectrophotometer
2. One absorbing solution with a UV absorbance of
approximately 1.00 AU.
a. Caffeine solutions or prednisone solutions are
suitable choices.
b. An absorbance of 0.90 to 1.10 AU is strongly
recommended.
3. DI or higher quality water
4. 12 test tubes
a. Vials are not recommended due to sample size.
b. Recommended sample size is 7 – 10 mL for UV
analysis.
c. Filters may be used at operator discretion.
Procedure
This procedure uses only line A1. There is no need to perform
this procedure on other lines. See the Carryover section in this
maintenance section for how to perform a carryover test, to
verify the rinse volume on all the lines.
1. Prime line A1 with no less than 20 mL using DI water.
2. Use the CD AutoPlus controls to obtain two samples
(blank 1, and blank 2) without removing the probe
from the DI water.
3. Round the theoretical dead volume up to the next
whole number.
4. Place the probe in the absorbing solution and rinse
the lines with the amount determined in step 3.
5. Use the CD AutoPlus controls to obtain a sample for
UV analysis.
6. Place the probe back in the DI water and prime the
system with 20 mL.
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