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3. In case carbon steel instruments are placed on the stainless steel
tray, the tray should be lined with a towel or paper wrap before
placing the instruments on the tray. There should be no direct
contact between the carbon steel and the stainless steel tray.
4. All instruments must be sterilized in an open position.
5. Use single-use wraps once only and discard after use.
6. Place a sterilization indicator strip on the tray.
7. Place instruments with ratchets opened and unlocked or clipped on
the first ratchet position.
8. Disassemble or sufficiently loosen multiple-part instruments prior
to packaging to permit the sterilizing agent to come into contact with
all parts of the instrument.
9. Tilt on edge items prone to entrap air and moisture, e.g.
hollowware, so that only minimal resistance to removal of air, the
passage of steam and condensate will be met.
10. Load items within the boundaries of the tray so that they do not
touch the chamber walls.
11. The operator may use racks to allow for adequate separation of
packaged instruments.
12. Load trays in such a way as to allow steam to move freely among
all items.
13. Once a week, use a biological spore test indicator in any load to
make sure sterilization is performed efficiently.
14. Make sure that all instruments remain apart during the sterilization
cycle.
15. Empty canisters should be placed upside-down, in order to prevent
accumulation of water.
11.2 Wrapped Instruments
1. Wrapped instruments should be packed in material that promotes drying
such as autoclave bag, autoclave paper, and muslin towels.