3.4
Dilution .......................................................................................................................... 17
3.5
WBC Measurement ......................................................................................................... 17
3.5.1 Measurement Principle: ...................................................................................... 17
3.5.2 WBC-Related Parameters ................................................................................... 18
3.5.3 HGB Measurement ............................................................................................ 20
3.6
RBC/PLT Measurement ................................................................................................... 20
3.6.1 Impedance Method ............................................................................................. 20
3.6.2 RBC-Related Parameters .................................................................................... 21
3.6.3 PLT-Related Parameters...................................................................................... 22
3.7
Wash .............................................................................................................................. 23
4.Software and Interface .................................................................................................................. 24
4.1. Login ................................................................................................................................ 24
4.2
Review ........................................................................................................................... 24
4.2.1 Trend Graph ....................................................................................................... 24
4.3
Calibration ...................................................................................................................... 25
4.3.1 Calibration Factors ............................................................................................. 25
4.3.2 Calibration with Calibrator ................................................................................. 25
4.4
Gain Setup ...................................................................................................................... 27
4.5
Performance ................................................................................................................... 28
4.5.1 Background Count ............................................................................................. 28
4.5.2 Reproducibility .................................................................................................. 28
4.5.3 Carryover .......................................................................................................... 28
4.6
Advanced Toolbox .......................................................................................................... 28
4.6.1 Export ............................................................................................................... 28
4.7
Software Update ............................................................................................................. 29
4.8
Status Indicator ............................................................................................................... 30
4.9
Buzzer ............................................................................................................................ 30
5 Fluidics ........................................................................................................................................ 31
5.1
Introduction to Fluidic Parts............................................................................................. 31
5.1.1 Valves ............................................................................................................... 31
5.1.2 LVM fluidic valve .............................................................................................. 32
5.1.3 Linkage Syringe Device ..................................................................................... 32
5.1.4 Vacuum pump .................................................................................................... 33
5.1.5 Air pump ........................................................................................................... 33
5.1.6 Sample probe ..................................................................................................... 34
5.1.7 Probe wipes ....................................................................................................... 34
5.1.8 Baths ................................................................................................................. 35
5.2
Sample Dilution Flow Chart ............................................................................................ 36
5.2.1 Whole Blood Mode ............................................................................................ 36
5.2.2 Pre-dilute Mode ................................................................................................. 36
5.3
Introduction to Fluidic Channels ...................................................................................... 37
5.3.1 WBC/HGB channel............................................................................................ 37
5.3.2 RBC/PLT channel .............................................................................................. 37
5.4
Sample Volume ............................................................................................................... 37