5 Maintenance
Sources
Agilent 6475 Triple Quad LC/MS System User Guide 69
Installing an Electrospray or Agilent Jet Stream source
1 Check that the appropriate capillary cap is installed.
2 Install the appropriate spray shield.
3 For Electrospray (ESI), do the following:
a Make sure the small hole is at the top ("12 o'clock position").
b If needed, use a T10 Torx screwdriver to loosen the two screws in the end
plate.
c Rotate the ESI spray shield clockwise until the hole is in the correct
position.
d Gently tighten the Torx screws again.
4 Install the spray chamber.
5 Close the spray chamber.
6 Connect the MultiMode cable delivering AJS nozzle voltage.
7 Connect the heater cable.
8 Connect the sample nebulizer gas tubing.
9 Connect the nebulizer sample line.
Do not hit the tip of nebulizer needle. The needle is not protected. If you
damage the needle, system performance can be affected.
10 Connect the reference nebulizer sample line and sample nebulizer gas tubing.
In the Data Analysis program,
1 In the Tune context, select the new ion source.
2 Turn on the LC/MS instrument.
3 While you wait for the set points to equilibrate, prepare and install calibrant for
the new ion source.
a Rinse a clean calibrant bottle with LC/MS-grade acetonitrile.
b Pour the ESI Calibrant into the calibrant bottle.
c Install the calibrant bottle into the calibrant delivery system.
To Next Page
Loading...
Need help?
General
