5 Maintenance
Sources
Agilent 6475 Triple Quad LC/MS System User Guide 70
When all set points are equilibrated:
1 In the Tune view or context, turn on the calibrant.
2 Purge the calibrant line for 5 minutes.
3 Do a calibration. If calibration is not available, do a check tune.
4 If the check tune results are not acceptable, run auto tune on a supported
tuning source.
5 When the instrument is ready, load or create the method to use with the ion
source.
6 Change the method settings for the new ion source.
Installing an APCI source
1 Install the appropriate spray shield.
2 Make sure the small hole is at the top ("12 o'clock position").
3 If needed, use a T10 Torx screwdriver to loosen the two screws in the end
plate.
4 Rotate the ESI spray shield clockwise until the hole is in the correct position.
5 Gently tighten the Torx screws again.
6 Install the spray chamber.
7 Close the spray chamber.
8 Close the spray chamber latch.
9 Connect the heater cable.
10 Connect the corona needle voltage cable.
11 Connect the sample nebulizer gas tubing.
12 Connect the nebulizer sample line.
In the Data Analysis program,
1 In the Tune context, select the new source.
2 Turn on the LC/MS instrument.
3 While you wait for the set points to equilibrate, prepare and install calibrant for
the new source.
a Rinse a clean calibrant bottle with LC/MS-grade acetonitrile.
b Pour the APCI Calibrant into the calibrant bottle.
c Install the calibrant bottle into the calibrant delivery system.
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