Applied Biosystems QuantStudio 7 Flex User Manual

A tab-delimited le (.txt) or Microsoft
™
Excel
™
le (.xls or .xlsx) that contains the
sample name and custom sample properties for a single experiment.
A separately licensed softw
are module that provides:
• System Security – Controls user access to the software. Provides a default
Administrator user account. You can dene additional user accounts and
permissions.
• Auditing – Tracks changes made to library items, actions performed by users,
and changes to the Security and Audit seings. The software automatically audits
some actions silently. You can select other items for auditing and specify the audit
mode. Provides reports for audited library items, Security and Audit changes,
and actions.
• Electronic Signature (e-signature) – Controls whether users are permied,
prompted, or required to provide a user name and password when accessing
certain software features. You can select which features are controlled and the
number of signatures required for access. When authorized persons use this
feature, they are creating a legally binding signature.
In the software, a numeric value that denes the sequence of quantities in the
standard curve. The serial factor and the starting quantity are used to calculate the
standard quantity for each point in the standard curve. For example, if the standard
curve is dened with a serial factor of 1:10 or 10X, the dierence between any two
adjacent points in the curve is 10-fold.
Regression coecient calculated from the regression line in the standard curve. The
slope indicates the PCR amplication eciency for the assay. A slope of -3.32
indicates 100% amplication eciency.
See also “amplication eciency (EFF%)“ on page 184 and “regression line“ on
page 190.
In the thermal prole, a group of one or more steps. Examples: PCR stage, cycling
stage (also called amplication stage), and hold stage.
A sample that you dilute and amplify along with unknown samples. This dilution
series can contain known starting quantities of the target of interest (absolute
standard curve) or it can be of known dilution factor (relative standard curve).
Following the run, the software interpolates the C
t
values of the unknowns to this
curve, yielding either specic quantities of the target (for absolute curves) or relative
quantities (for relative dilution curves).
See also “standard curve“ on page 191.
In standard curve and relative standard curve experiments:
• The best-t line in a plot of the C
t
values from the standard reactions ploed
against standard quantities. See also “regression line“ on page 190.
• A set of standards containing a range of known quantities. Results from the
standard curve reactions are used to generate the standard curve. The standard
curve is dened by the number of points in the dilution series, the number of
standard replicates, the starting quantity, and the serial factor.
sample definition
file
security, auditing
and e-signatur
e
serial factor
slope
stage
standard
standard curve
Limited product warranty
QuantStudio
™
6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide
191

Applied Biosystems QuantStudio 7 Flex Specifications

General

Brand	Applied Biosystems
Model	QuantStudio 7 Flex
Category	Laboratory Equipment
Language	English