Biotage
®
Selekt User Manual | © Biotage 2020
Contents
Biotage
®
Selekt
User Manual
CONTENTS
System Overview
1 Columns
1 Racks and Vessels
2 Solvent Supply
2 Internal UV Detector
2 External Detector
2 Collection and Fractionation Methods
3 E-mail Functionality
3 Audible Alarm
3 Lighting
3 Biotage
®
Safety Valve
3 Spektra Software License (Optional)
Prepare the System
4 Mount Column Holders
4 Install Biotage
®
Safety Valve (Optional)
5 Mount a Biotage
®
SNAP XL Column (Optional)
6 Inspect the Tubing
6 Start Up the System
7 Setup of Automatic Conversion
Between Normal and Reversed Phase
7 Assign Solvents and Set Reservoir Volumes
7 Prime the Solvent Inlets
7 Flush the System
Set Up a Purification
8 Specify the Gradient
10 Specify the Information Parameters
10 Specify the Column Parameters
11 Specify the Collection Parameters
11 Specify the Rack Parameters
Start, Monitor, and Control a Purification
13 Start a Purification
13 Monitor the Purification in Progress
14 Manual UV Zero
14 Start and End an Isocratic Segment
15 Add, Empty, and Replace Racks During the Run
16 Manually Fractionate
16 Line Flush, Decompression, and Detector Flush
16 Auto Extend
16 Pause, End, or Abort a Purification
16 Edit a Purification
17 Confirm Cleanup
Unload a Purification
18 Flush the Column with Air
18 Purge the Column
18 Unload the Run
18 Flush the System
18 Shut Down the System
Results
19 Search the Results
20 Analysis of the Result
21 Customize the Report
21 Add a Report Note
21 Export the Report
22 Base a New Run on a Previous Run
Flushes and Purge
23 Flush
23 Flow Paths
24 Air Flush
24 Purge
Data Administration
25 Administrate Column Types
25 Administrate Solvents
26 Administrate Racks
26 Administrate Results
26 Administrate User Accounts
System Settings
27 System
27 Purification
28 Optional Detectors
28 Network
29 Licenses
Maintenance
30 Back Up and Restore the System’s Database
30 Export System Logs
30 Calibrate the Internal UV Detector
31 Clean the Exterior of the System
31 Clean the Flow Cell of the Internal UV Detector
32 Clean or Release Check Valves
32 Leaks
34 Replace the Fuses
34 Replace the Needle
34 Sonicate Solvent Inlet Filters
Troubleshooting
35 Fraction Collector-Related Problems
35 Gradient Problems
35 Internal UV Detector-Related Problems
36 Leak Detected
36 Overpressure Detected
36 Pump-Related Problems
37 QR Reader Problems
37 Contact Biotage
®
1-Point Support
™
Solvent Specifications
General Information
39 Consumables and Accessories
39 Manufacturer
39 Contact Us
Notes