Biotage
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Selekt User Manual | © Biotage 2020
Solvent Specifications
Solvent Specifications
Warning
»
Many solvents are considered hazardous to humans and the environment, so take appropriate safety precautions when using them.
Comply with Safety Data Sheets (SDS) and any other applicable regulations for the safe use, handling, transporting, storage, and
disposal of these solvents.
Solvent CAS No. Strength
1,2
Selectivity Class
3
UV Cutoff
(nm)
Vapor Pressure at
20°C (psi)
Vapor Pressure at
20°C (mbar)
Acetone 67-64 -1 0.56 6 (V Ia) 330 3.6 247.4
Acetonitrile 75-05-8 0.65 6 (VIb) 190 1.4 93.6
Cyclohexane 110- 82-7 0.04 0 210 1.5 103.4
Dichloromethane (DCM) 75-09-2 0.42 5 (V) 235 6.9 475.3
Ethanol 64 -17-5 0.88 2 (II) 210 1.3 90.0
Ethyl acetate 141-78- 6 0.58 6 (V Ia) 255 1.4 98.3
n-Heptane 142-82-5 0.01 0 210 0.7 47.4
n-Hexane 110-54-3 0.01 0 210 2.3 161.6
Methanol 67-56-1 0.95 2 (II) 210 1.9 129.7
2-Propanol (IPA,
isopropanol)
67-63-0 0.82 2 (II) 210 0.6 44.0
Tetrahydrofuran 109-99-9 0.57 3 (III) 220 2.5 172.4
Toluene 108-88-3 0.29 7 (VII) 286 0.4 29.1
Water 7732-18-5 1.00
*
0 190 0.3 23.4
Table 4. Solvent specifications.
1
Neue U. D. HPLC Columns Theory, Technology, and Practice, Wiley-VCH (1997).
2
Dean J. A. Lange’s Handbook of Chemistry, 15th edition, McGraw-Hill (1999).
3
Snyder L. R. and Kirkland J. J. Introduction to Modern Liquid Chromatography, Wiley (1979).
*
When water is used in reversed phase chromatography, the strength value is 0.