Biotage Selekt - Collection and Fractionation Methods; External Detector; Internal UV Detector; Solvent Supply

 Biotage
®
Selekt User Manual | © Biotage 2020
System Overview
Solvent Supply
The system is equipped with four solvent inlets found on the
right side of the system (S1-S4).
Secondary Solvent Containment with
Biotage
®
Solvent Detector
*
A maximum of four 5-L reservoirs can be placed on the optional
secondary solvent containment; see Figure 4. Larger reservoirs
than 5 L must be placed elsewhere.
Note: To be compliant with the US secondary containment
regulations, do not use reservoirs larger than 4 L.
Figure 4. The optional secondary solvent containment.
Solvent and Waste Monitoring
The system can maintain a running calculation of the fluid levels
in the reservoirs based on information entered by the user.
With solvent and waste monitoring enabled, the system will:
» Inform the user when there is not enough
solvent or waste capacity for a run.
» Issue an on-screen notification when a solvent
level is below 20% of the set capacity.
» Issue an on-screen notification when the waste
level is above 85% of the set capacity.
» Pause the system when it is time to refill a
solvent reservoir (when 10% is left) or empty
the waste reservoir (when 95% full).
Instrument Tray with Biotage
®
Solvent Detector
*
An optional instrument tray with a solvent detector is available
for safe unattended operation. When solvent is detected, the
system is paused and all pump functionality disabled until the
solvent detector is dry again.
Figure 5. The optional instrument tray with a solvent detector.
Internal UV Detector
The system includes either a 200 to 400 nm UV detector or
a 198 to 810 nm UV-VIS detector.
External Detector
It is possible to connect an external detector with analog
output signal. For more information, see the Biotage
®
Selekt
Installation and Safety document (P/N 416182).
Collection and Fractionation Methods
Available detection signals are:
» λ-All: The system uses average absorbance
within a user-defined wavelength range for
collection and fractionation. Only available on
systems with a Spektra software license.
» UV1 and UV2: The system uses one or two wavelength
signal(s) for collection and fractionation.
» EXT: The system uses the signal from an external
detector for collection and fractionation. Optional.
Possible collection and fractionation parameters are
Collect All, Threshold, and Valley Fractionation. The system
always fractionates on volume, which is when a test tube or
bottle reaches the specified vessel volume or a user-defined
maximum fraction volume for the run. At any time, you can
manually switch to a new collection vessel by pressing
in
the chromatogram.
Any signal combination can be used for collection and
fractionation. Signals that are not used for collection can be
used for monitoring.
Figure 6. The Collection panel expanded on a system with a Spektra
software license and an external detector connected.
*
It is only possible to connect one Biotage Solvent Detector to the system.