Biotage
®
Selekt User Manual | © Biotage 2020
Prepare the System
Mount Column Holders
The system is shipped with column holders for Sfär columns
of different sizes. Ensure that they are mounted in the desired
positions on the system.
Figure 9 shows how to mount a column holder on the right side
of the system. There are also two positions for column holders
on the front of the system that can be used for Biotage columns
up to 50g; see Figure 10.
Figure 9. Slide the column holder screw into the desired position on
the right side of the system and then place the holder over the screw
and fasten.
Figure 10
. Biotage columns up to 50g can be placed on the front of
the system.
Install Biotage
®
Safety Valve (Optional)
Warning
»
Always use Biotage Safety Valve (P/N 417115SP) when
processing columns or combinations (e.g. column and dry
load vessel) with a total CV larger than 0.8 L.
»
Never use columns or combinations with a total CV larger
than 3.1 L.
»
Do not over-tighten the fittings or the tubing may become
damaged.
The optional safety valve (P/N 417115SP) enables you to use
columns or combinations (e.g. column and dry load vessel)
with a combined column volume larger than 0.8 L and up to
a maximum of 3.1 L.
The safety valve is designed to open at 8 bar and discharge
fluid until the pressure drops below 8 bar.
Prepare the System
Required tools:
»
Torx T6 screwdriver.
»
Open-end wrench, 14 mm.
»
Open-end wrench, 17 mm.
To install the safety valve:
1. Fit the supplied drain tube to the outlet of the safety valve
(see A in Figure 11) using the two open-end wrenches.
2. Mount the safety valve to the right side of the system:
a. Slide the valve into one of the rails on the right side of
the system; see Figure 11.
b. When the safety valve is in the desired position,
tighten the fastening screw (see B in Figure 11) using
the Torx T6 screwdriver.
3. Connect the tubing leading from the C1 or C2 port on the right
side of the system (see Figure 15 on page 6), depending
on which channel you want to use, to the connection at the
bottom of the safety valve (see C in
Figure 11
).
4. Connect the extra tube supplied with the safety valve to the
connection at the top of the valve (see D in Figure 11).
Note: This tube is to be connected to the Luer fitting at
the top of the column. For instructions on how to mount
a SNAP XL column onto the system, see “Mount a Biotage
®
SNAP XL Column (Optional)” on page 5.
5. Insert the drain tube into the system’s waste reservoir.
Figure 11
DD
AA
CC
BB
. The safety valve. A: Drain tube. B: Screw for fastening the
valve to the system. C: Tube from the C1 or C2 port on the right side of
the system. D: Tube going to the top of the column.
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