Biotek EL800 User Manual

Operator’s Manual
Absorbance Microplate Reader
ELx800
™

Biotek EL800 Specifications

General

Brand	Biotek
Model	EL800
Category	Laboratory Equipment
Language	English

Summary

Document Conventions

Intended Use, Quality Control, and Shipping

Safety Warnings and Precautions

Electrical and Grounding Warnings

Internal Voltage and Liquid Hazards

Chapter 1: Introduction

Introducing the ELx800 Absorbance Microplate Reader

Hardware Features

Instrument Specifications

Provides technical specifications for the ELx800.

Chapter 2: Installation

Unpacking and Inspecting the ELx800

Instructions for safely unpacking the instrument.

Setting Up the ELx800

Guides on configuring the ELx800 for operation.

Setting Up Serial Port Communication

Repackaging and Shipping the ELx800

Procedures for safely repackaging and shipping the instrument.

Chapter 3: Operation

System Startup

Main Menu Navigation

Assay Definition Parameters

Defines assay parameters like method, map, formula, and curve fit.

Defining METHOD

Defining MAP

Sets up plate layout for samples, blanks, and controls.

Defining FORMULA

Creates formulas for data calculation and validation.

Defining CURVE

Configures curve-fitting parameters for assays.

Reading a Microplate

Procedures for performing a plate read.

Selecting an Assay to Run

Printing Reports

Options for printing assay results and data.

Editing Standard Outliers

Using the Utility Options

Accesses instrument setup and configuration settings.

Selecting Read Options

Chapter 4: Instrument Qualification

Recommended Qualification Schedule

Outlines recommended intervals for instrument testing.

Qualification Procedures

Details the steps for verifying instrument performance.

System Test Procedure

Performs an automated self-test of the instrument.

Absorbance Plate Test

Tests mechanical alignment and optical performance using a test plate.

Liquid Testing

Confirms performance using liquid samples.

Liquid Test 1

Chapter 5: Maintenance and Decontamination

Instrument Maintenance

Routine Cleaning Procedure

Replacing the Bulb

Instructions for replacing the instrument's lamp.

Decontamination Procedure

Steps for decontaminating the instrument for safety.

Chapter 6: Troubleshooting and Error Codes

Error Codes

Lists and explains instrument error codes.

General Errors

Details non-fatal error conditions and their causes.

Fatal Errors

Lists critical errors requiring immediate attention.

Appendix A: Computer Control

Controlling the Reader With Gen5

Setup and operation of Gen5 software with the reader.

Controlling the Reader With KC4

Controlling the Reader With KCjunior

Appendix B: Using 384-Well Geometry

Perform 384-Well Plate Read in Gen5

Perform 384-Well Plate Read in KC4

Perform 384-Well Plate Read in KCjunior

Appendix E: Instructions for Programming a New Assay

Programming the ANA Screen Enzyme Immunoassay Kit

Programming the Human Anticardiolipin IgG Enzyme Immunoassay Kit