Liquid Testing | 117
ELx800 Operator's Manual
Note: 96-well, flat-bottom microplates are required for the
liquid tests (Corning Costar
®
#3590 is recommended). Use
new microplates; any fingerprints or scratches may cause
variations in readings.
IMPORTANT: Before running the liquid tests, ensure that
the reader is not running in Rapid Mode. To check this,
select UTIL READ and then cycle through the prompts
until READ IN RAPID MODE? is displayed. Choose NO for
an accurate result.
Liquid Test 1
1. Using freshly prepared stock solution A or B (the concentrated solution),
prepare a 1:2 dilution using DI water (one part stock, one part DI water; the
resulting solution is a 1:2 dilution).
2. Pipette 200 μl of the concentrated solution into Column 1 of the plate.
3. Pipette 200 µl of the diluted solution into Column 2 of the plate.
After pipetting the diluted test solution into the microplate and before
reading the plate, we strongly recommend shaking the plate for four
minutes. This will allow any air bubbles in the solution to settle and the
meniscus to stabilize. If a plate shaker is not available, wait 20
minutes after pipetting the diluted test solution before reading the
plate.
4. Read the microplate five times at 405 nm using the Normal Read Mode
(
Chapter 3), single wavelength, no blanking.
5. Print the five sets of raw data or export it to an Excel spreadsheet using
Gen5™, KC4™, or KCjunior™.
If you are exporting the data to your own Excel spreadsheet, perform
the calculations described below and keep the spreadsheet for future
tests.
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