44 | Chapter 3: Operation
BioTek Instruments, Inc.
Define
The Main Menu option DEFINE allows you to define the data acquisition and reduction
parameters for a new assay, or modify previously defined assays stored in memory.
1. Start at the Main Menu and select DEFINE to display the SELECT ASSAY
NUMBER screen.
S E L E C T ASSAY NUMBER:0 1
N A M E : H B S -A G 1
2. Select an assay to define or modify, and then press ENTER. See Selecting an
Assay to Define
on page 46 for detailed instructions. The EDIT ASSAY NAME
screen will appear.
If you are modifying/selecting a PANEL assay (#99), see page 83 for
instructions.
N A M E : H B S -A G 1
- / : SP A C E
3. (Optional) Edit the assay name, and then press ENTER. See Editing the Assay
Name
on page 46 for detailed instructions. The DEFINE menu will appear:
D E F I N E :
M E T H O D MAP FORMULA CU R V E
The following options are available within the DEFINE menu:
•
METHOD: Define the wavelength type (single or dual), wavelength(s), and
plate geometry (page 47).
•
MAP: Specify the plate layout, using blanks, controls, standards, and/or
samples. Choose to map the plate manually, or let the software map it
automatically (page 51).
•
FORMULA: Define cutoff, transformation, and validation formulas. Create
variables to be used within formulas (page 67).
•
CURVE: Specify a curve fit type and x/y axis types (lin/log). Specify whether
or not standard outliers can be edited, and then the method by which they will
be edited. Enable or disable the extrapolation feature (page 79).