Biotek EL800 - Requirements; Figure 18: Sample Standards Certificate for the Absorbance Test Plate

110 | Chapter 4: Instrument Qualification
BioTek Instruments, Inc.
Requirements
To run the Absorbance Plate Test, you need BioTek's Absorbance Test Plate
(PN 9000547 or PN 7260522), with its accompanying Standards Certificate, shown
in
Figure 18. Figure 19 shows a sample Test Plate Analysis Report.
This test plate can be used for testing the reproducibility, linearity, and alignment of
your BioTek autoreader. The following calibration data has been recorded by a
N.I.S.T. traceable spectrophotometer.
WAVELENGTH (nm)
Well 405nm 450nm 490nm 550nm 620nm 630nm 690nm 750nm
C1 0.147 0.140 0.135 0.130 0.136 0.136 0.127 0.134
E2 0.618 0.575 0.574 0.568 0.573 0.568 0.485 0.434
G3 1.133 1.052 1.051 1.040 1.050 1.040 0.881 0.783
H6 1.701 1.578 1.577 1.560 1.575 1.560 1.323 1.179
F5 2.279 2.024 1.976 1.956 1.893 1.865 1.537 1.272
D4 2.945 2.604 2.545 2.513 2.437 2.400 1.972 1.632
Set # 2453 Serial # 161259
Figure 17: Sample Standards Certificate for the Absorbance Test Plate
Alignment Results:
WELLS B2 B12 G1 G11
DATA 0.000 0.000 0.000 0.000
TOLERANCE 0.015 0.015 0.015 0.015
RESULT PASS PASS PASS PASS
Wavelength = 405 nm
Accuracy Results:
WELLS C1 E2 G3 H6 F5 D4
STANDARD 0.147 0.618 1.133 1.701 2.279 2.945
MIN. VALUE 0.124 0.586 1.090 1.647 #N/A #N/A
MAX. VALUE 0.170 0.650 1.176 1.755 #N/A #N/A
DATA 0.148 0.616 1.129 1.695 2.243 2.850
RESULT PASS PASS PASS PASS #N/A #N/A
Repeatability Results:
WELLS C1 E2 G3 H6 F5 D4
READ1 0.148 0.616 1.129 1.695 2.243 2.850
MIN. VALUE 0.137 0.600 1.108 1.668 #N/A #N/A
MAX. VALUE 0.159 0.632 1.150 1.722 #N/A #N/A
READ2 0.149 0.616 1.130 1.695 2.242 2.853
RESULT PASS PASS PASS PASS #N/A #N/A
Figure 18: Sample Test Plate Analysis Report
 Note: The Test Plate Analysis report varies slightly depending on how it
was run (via the keypad, Gen5™, KC4™, or KCjunior™).
 Important! The ELx800’s Absorbance Plate Test tests the accuracy and
repeatability specifications from 0.000 to 2.000 OD only. In the example
above, the Analysis report displays the OD values read in well positions
D04 and F05 but does not indicate PASS or FAIL, because the values are
higher than 2.000 OD and therefore are not within the test range.
S A M P L E