Liquid Testing | 123
ELx800 Operator's Manual
Liquid Test 3 (For “UV” Models Only)
Liquid Test 3 is an optional test offered for sites that must have proof of linearity
at wavelengths lower than those attainable with the Absorbance Test Plate. This
test verifies operation of the ELx800™ at 340 nm and is optional because the reader
has good “front-end” linearity throughout its wavelength range.
Required Materials:
• 340 nm filter installed in the reader
• New 96-well, flat-bottom microplates (Corning Costar
®
#3590 is
recommended)
• Calibrated hand pipette(s)
• Beakers and graduated cylinder
• Precision balance with readability to 0.01 g
• Buffer solution A
or B
Solution A: 10x Concentrate Phosphate Buffered Saline (PBS)
Required Materials:
• Deionized water
• Ingredients shown in
Table 4
• D-NADH powder (D-Nicotinamide Adenine Dinucleotide, Reduced
Form) Sigma
®
bulk catalog number N 8129, or preweighed 10-mg vials,
Sigma
®
number 340-110
Store the D-NADH powder according to the guidelines on its packaging.
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