122 | Chapter 4: Instrument Qualification
BioTek Instruments, Inc.
Alignment Test
1 Using the prepared plate, conduct a turnaround test by reading the
plate with the A1 well in the H12 position five times. This test results in
values for the four corner wells that can be used to determine
alignment.
2 Calculate the means of wells A1 and H1 in the normal plate position
(data is from Linearity Test) and in the turnaround position (from Step
1 above). Compare the mean reading for well A1 to its mean reading
when in the H12 position. Next, compare the mean values for the H1
well to the same well in the A12 position. The difference in the values
for any two corresponding wells should be within the accuracy
specification for the instrument.
For example:
If the mean of well A1 in the normal position is 1.902, where the
specified accuracy is ± 1.0% ± 0.010 OD, then the expected range for the
mean of the same well in the H12 position is 1.873 to 1.931 OD.
(1.902 * 1% = 0.019 + 0.010 = 0.029, which is added and subtracted from
1.902 for the range.)
3 If the four corner wells are within the accuracy range, the reader is in
alignment.
Accuracy Specifications:
For comparison in this test, the following accuracy specification is
applied, using Normal Mode and a 96-well microplate:
± 1.0% ± 0.010 OD from 0.000 to 2.000 OD @ 405 nm
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