8 | Chapter 1: Introduction
BioTek Instruments, Inc.
• Optical specifications for the 340 to 400 nm range (30-second read):
Absorbance Measurement
Range:
0.000 to 2.000 OD
Accuracy: ± 2.5% ± 0.020 OD from 0.000 to 2.000 OD
@ 340 nm
Linearity: ± 2.5% from 0.000 to 2.000 OD @ 340 nm
Repeatability (STD): ± 2.0% ± 0.010 OD from 0.000 to 2.000 OD
@ 340 nm
Narrow Beam/NB Model
Filters:
10 nm half-bandwidth interference filters.
User-accessible filter wheel. Up to 5 filters may
be installed on the instrument at one time.
Filters supplied: 405 nm, 450 nm, 490 nm and
630 nm.
The following specifications apply to 96-well, flat- or round-bottom plates with a
50-second read (normal read mode).
Absorbance Measurement
Range:
0.000 to 3.000 OD
Accuracy: ± 1.0% ± 0.010 OD from 0.000 to 2.000 OD
@ 405 nm
Linearity: ± 1.0% from 0.000 to 2.000 OD @ 405 nm
± 3.0% from 2.000 to 3.000 OD @ 450 nm
Repeatability (STD): ± 0.5% ± 0.005 OD from 0.000 to 2.000 OD
@ 405 nm
The following specifications apply to 96-well, flat- or round-bottom plates with a
30-second read (rapid read mode).
Absorbance Measurement
Range:
0.000 to 3.000 OD
Accuracy: ± 2.0% ± 0.020 OD from 0.000 to 2.000 OD
@ 405 nm
Linearity: ± 2.0% from 0.000 to 2.000 OD @ 405 nm
Repeatability (STD): ± 1.0% ± 0.010 OD from 0.000 to 2.000 OD
@ 405 nm