88 | Chapter 7: Troubleshooting
BioTek Instruments, Inc.
Performance Issues
Issue Solution
Fluid stream missing wells
Check Tip Holder, make sure it is properly seated in
the Dispense Arm.
Select the correct Plate Type.
Tips are clogged. (See Unclogging Tips in the
Preventive Maintenance chapter.)
Fluid splashing out of the wells
Select the correct Plate Type.
Reduce the Flow Rate (only available when defining a
Protocol, not a Quick Dispense).
Lower the Dispense Height.
Uneven dispensing
Make sure all cassette components are properly
seated in their respective positions.
Tips are clogged. (See Unclogging Tips in the
Preventive Maintenance chapter.)
Recalibrate the cassette.
Replace the tubing. (On the Operator’s Manual CD,
see 7171017_(current Rev)_Replacing the
tubing_8x14.PDF
)
Dispenser skipping columns
Check/define the Plate Map
(uPlate).
Tips clogging
Filter the dispense fluid to 50 microns before
dispensing.
Replace the tubing.
Viscous fluids sticking to tips
Vary the Flow Rate: experiment with different flow
rates to determine which setting best forces fluid to
break from the tip.
Clean the Tips (See the Preventive Maintenance
chapter.)
Cannot communicate with
computer
Check the cabling (See next page.)
Select the correct COM Port
Turn on dispenser; display Main Menu
To Next Page
Loading...