Biotek MicroFlo select - Dispense Precision and Accuracy Tests

Dispense Precision and Accuracy Tests | 73
MicroFlo Select Microplate Dispenser
Dispense Precision and Accuracy Tests
Overview
Dispense precision is a measure of the variability of volumes dispensed from tube to
tube across the manifold. The optical density of the solution in a well is proportional to the
total volume of the solution in the well. If the % Coefficient of Variation (%CV) is
calculated, the result is a measure of the uniformity of the distribution of dispensed
volumes across the manifold. It is the ratio, expressed in percent, of the standard deviation
of the distribution of fluid volumes in the wells to the mean value of volume per well. The
uniformity of distribution across the manifold improves as the %CV is lowered.
Dispense accuracy is a measure of the average volume dispensed per well. It is
independent of precision. The volume per well may vary greatly over a plate, yet the
accuracy may be exact because it is an average of the volumes.
The accuracy specifications for the cassettes are intended to describe the performance of
tubing throughout its life. Typically, achieving a dispense accuracy that is 1/10
Th
of the
specified accuracy is the goal of calibration.
Dispense accuracy is computed gravimetrically using this formula:
ected
ectedactual
m
mm
Accuracy
exp
exp
)(
100(%)


Dispense Precision and Accuracy
Cassette Precision Accuracy
< 10%CV @ 1L per well ± 10% @ 1L per well
1µL
< 5%CV @ 2L per well* ± 5% @ 2L per well*
< 10%CV @ 1L per well ± 10% @ 1L per well
1µL 1536
< 5%CV @ 2L per well* ± 5% @ 2L per well*
< 5%CV @ 5L per well ± 4% @ 5L per well
5µL
< 2.5%CV @ 10L per well* ± 2% @ 10L per well*
< 4%CV @ 10L per well ± 4% @ 10L per well
10µL
< 2%CV @ 20L per well* ± 2% @ 20L per well*
 * These specifications are for these dispense volumes and higher.
 Note: The above specifications are for performance. For IQ/PQ/OQ
purposes, we have added 1.0% additional tolerance to the %CV to
accommodate various test solutions, off-peak wavelengths, reader
errors, and pipette errors.