Flush the Tubing Cassette | 59
MicroFlo Select Microplate Dispenser
Flush the Tubing Cassette
Daily Maintenance involves priming the tubing with an appropriate reagent at the
beginning of the day, and, flushing the tubing to effectively remove all contaminants at
the end of the day.
The type of rinse fluid to use is determined by the type of fluid you are dispensing.
Some dispense fluids require the use of enzyme-active detergent, buffered saline,
ethanol or isopropyl alcohol, rather than deionized water alone.
Tools and Supplies
Deionized or distilled water
Buffered saline solution or enzyme-active detergent for protein or cell based
assays
At the start of the day:
Prime the tubing to prepare for a dispense run.
1. Reload the cassette and fill the supply vessel:
When dispensing solutions not effected by water, simply prime with the
dispense fluid.
When dispensing protein solutions, first prime the tubing with a
buffered saline solution to remove any traces of water in the tubing,
then, prime with the dispense fluid.
2. Hold the Prime button on the keypad until fluid flows into the priming trough
and all visible air bubbles have been removed.
0.
At the end of the day:
Purge the tubing to reclaim the dispense fluid, then Prime the tubing to flush it clean.
1. Hold the Purge button on the keypad until the tubing appears empty.
2. Replace the supply vessel with the appropriate rinse fluid:
When dispensing water soluble solutions use DI water.
When dispensing protein solutions, first prime the tubing with a
buffered saline solution to remove protein particles, then, prime with DI
water.
3. Hold the Prime button on the keypad:0.
1µL cassette = 5 seconds
5µL cassette = 12 seconds
10 µL cassette = 6 seconds.
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