Biotek MicroFlo select - Performing the Precision and Accuracy Tests

Performing the Precision and Accuracy Tests | 77
MicroFlo Select Microplate Dispenser
Performing the Precision and Accuracy Tests
Prerequisite:
 Prepare the test solutions as described on page 76.
 Make a copy of the applicable worksheet beginning on page 80.
Procedure:
1. Install the cassette to be tested.
2. Turn on the MicroFlo Select and make sure the cassette type setting is correct.
3. Turn on the balance.
4. Fill a beaker or other vessel with the test solution.
5. Define a Protocol and save it for reuse. Set the parameters based on the desired
test volume:
 Set the
Dispense Volume to match the Test:
 Set the
Flow Rate to:
 1 µL cassette = Medium
 5 µL cassette = High
 10 µL cassette = High
 Retain the default settings for Dispense Height, Plate Clear Height and
Horizontal Position.
 Define a
Prime before start: set the volume to 10 µL.
 Set the
Number of Pre-dispenses to 2.

1536 Test only: : Change the plate map to dispense to every other column.
It will be easier to observe the dispense results than dispensing to the whole
plate.
6. Place a clean/new microplate on the balance and tare the balance.
Test Volume
1 µL 1 µL
5 µL 5 µL
10 µL 10 µL
50 µL 50 µL
100 µL 100 µL
1536 6 µL