34 | Essential Concepts
BioTek Instruments, Inc.
Remember, when defining a run: dispense volume is per tube, not
per well. Divide the desired volume by the number of tubes in a well.
Test Tube Racks
The MicroFlo supports two types of test tubes that fit in a test tube rack (PN 7170520)
available from BioTek as an accessory item:
20 - 12 mm X 75 mm, 6 mL capacity tubes. Select
75T when using the
keypad.
20 – 13 mm X 100 mm, 9 mL capacity tubes. Select
100T when using the
keypad.
Before dispensing to a test tube rack, the odd-numbered dispense tubes must be
removed from either the fluid supply or from the cassette itself. Only the even-
numbered channels are properly positioned to dispense to the tubes.
Remove channels 1, 3, 5, and 7 from the cassette or fluid
supply before dispensing to test tubes.
You must also remove the plate carrier and replace it with the test tube rack. Follow
the instructions to Install the Test Tube Rack in
Appendix B.
Plate Type Columns/Rows Tubes per Well Tubes removed
20 75mm Tubes 5 x 4 1 1, 3, 5, 7
20 100mm Tubes 5 x 4 1 1, 3, 5, 7
If you regularly use the special plates or test tubes that require some of the
dispense tubes to be removed from the fluid supply before dispensing, you
should consider dedicating certain cassettes for the purpose. Removing the
unused tubes from the cassette, rather than from the fluid supply, will preserve
them for future use and make the cassette easier to handle. Review the
instructions on the operator’s manual CD titled: 7171017_(current
Rev)_Replacing the tubing_8x14.PDF to learn how to remove the tubes.
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