Biotek MicroFlo select - Procedure

Calibrating the Tubing Cassette Gravimetrically | 101
BioTek Instruments, Inc.
Figure 1: A MicroFlo Select in preparation for the gravimetric cassette calibration, and a digital
precision balance with 0.0001 g resolution.
Procedure:
1 Install the cassette to be calibrated.
2 Turn on the MicroFlo Select and make sure the cassette type setting is correct.
3 Turn on the balance.
4 Define a Protocol with the following parameters:
 Set the
Dispense Volume to match the cassette:
 Set the
Flow Rate to:
 1 µL cassette = Medium
 5 µL cassette = High
 10 µL cassette = High
 Retain the default settings for Dispense Height and Horizontal Position
 Define a
Prime before start: set volume to 10 µL.
 Set the
Number of Pre-dispenses to 2.
Cassette Volume
1 µL 10 µL
5 µL 50 µL
10 µL 100 µL