100 | Gravimetric Calibration Instructions
MicroFlo Select Microplate Dispenser
Calibrating the Tubing Cassette Gravimetrically
To ensure precise and accurate dispensing, the tubing cassette must be calibrated. This
procedure is one of several options for meeting this requirement. You can purchase
calibrated cassettes from BioTek, rather than recalibrate a used cassette. Or, you can
purchase a MicroFlo accessory from BioTek: the Calibration Kit, PN 7170017.
This procedure can also be used to meet the monthly requirement to “Verify
Performance.”
This gravimetric method takes an experienced lab tech about 45-60 minutes to complete.
It is an iterative process of measuring and adjusting until the desired accuracy is
achieved.
Before you begin, learn “About Calibrating Cassettes Gravimetrically” on
page 105.
These instructions are based on dispensing water to calibrate the tubing, which is
sufficient for most applications. Adjust the formulas used in this method to
accommodate a different test fluid, if desired.
Recommended: Allow the cassette to equilibrate to ambient room
conditions for at least 2 hours prior to calibration.
Required Materials:
A supply vessel containing the deionized (DI) water, with a little dye, if desired, to
make it easier to see the fluid.
96-well, 1x12 strip microplate shipped with the dispenser (Greiner Bio-One, Cat.
No. 701070 or equivalent).
Precision digital balance with a capacity of 1000 g and readability of 0.0001 g
resolution. If unavailable, perform multiple dispenses to sufficiently increase the
dispense weight.
MicroFlo Select.
Gravimetric Cassette Calibration Worksheet: two formats are offered, an Excel
®
worksheet is provided on the Operator’s Manual CD in the Spreadsheets folder
and a tabular form can be found on page 104.
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