78 | Instrument Qualification
BioTek Instruments, Inc.
7. Put the Tube Organizer into the test fluid vessel and Prime the tubing until
any large air bubbles are removed.
8. Run the dispense protocol.
9. Place the plate on the balance and record the Total Dispense Weight in the
worksheet.
10. Using a calibrated hand pipette, add the specified amount of de-ionized water
to each well to raise the fluid level to approximately 150 L, except for the 1536
Test:
0.
11. Read the plate in an absorbance reader using the dual-wavelength method:
read the plate at 630 nm and 450 nm.
If you are using one of BioTek’s keypad-based absorbance readers,
such as the ELx800™ or ELx808™, ensure that the reader is not
running in Rapid mode. To check this, select UTIL > READ and cycle
through the prompts.
12. Calculate the Delta OD: (630nm – 450nm), Mean Absorbance, Standard
Deviation
, and the %CV for the wells under test. %CV = (Standard Deviation
Mean) * 100.
13. Print the report, obtain required signatures, and store it according to regulatory
guidelines.
0.
If one or more of your tests are failing, make sure the dispense tubes are not clogged,
(follow the instructions for Unclogging the Tips in the
Maintenance chapter). If that
doesn’t work, recalibrate the cassette and repeat the test(s). If your tests continue to
fail, contact BioTek’s Technical Assistance Center (TAC).
Test Volume
1 µL 150 µL
5 µL 150 µL
10 µL 100 µL
50 µL 100 µL
100 µL 50 µL
1536 0 µL
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