44 | Operation
BioTek Instruments, Inc.
Dispense Protocol Parameters
Dispense Parameters
Name and
Number
Using the keypad: When creating a protocol onboard using
the keypad, the MicroFlo assigns the first available number
to newly created protocols. You can assign a descriptive
name, but protocols are selected for editing and running by
their number. Learn more, including how to enter a name on
page 45.
Using the LHC: When creating a protocol using the LHC,
enter a unique name in the Protocol Name field. And, while
they can be different, BioTek recommends using the same
name when saving the protocol file. The file will be saved
with a .LHC filename extension.
Plate Using the keypad: Follow the µPlate instructions on page
51.
Using the LHC: refer to the LHC’s Help for instructions.
Dispense
Volume
Enter the amount of fluid in microliters (µL) to dispense to
each well. (See also: Special Plate Handling, 12-, 48-well
plates and test tubes in the Essential Concepts chapter.)
Flow Rate Low, Medium, or High:
Low rate is recommended when using PCR or round-bottom
plates to avoid fluid splashing.
High rate is recommended for faster throughput.
Dispense Height The distance between the bottom of the well and the
dispense tips when dispensing.
Plate Clearance
Height
The height the dispense arm moves to after dispensing to a
column and while it moves to the next column.
The height is set in steps from 0 to 2048. Refer to the
manufacturer’s specifications when setting the height for
non-standard vessels. Always keep the tips at least 2 mm
above the surface.
Use the two height settings to position the tips inside the
wells when dispensing, and raise the tips safely above the
plate during transitions. Angled dispensing and lowering the
tips into the wells may minimize disturbance to cell layers in
applicable assays.
Using the keypad: Press Clear to restore default values for
the current plate type. To adjust the height, enter the
approximate number of steps, assess the value in
millimeters, and adjust accordingly.
Using the LHC: Click Show advanced options and select the
button to “Use the default” height or choose a custom
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