cytiva BPG Columns - Page 73

Test chromatogram
T
he following test chromatogram shows the parameters used to calculate peak broad-
ening and peak symmetry.
V
e
V
h
a b
50%
10%
y
x
W
h
The description of the axis are:
x
= Volume or time
y = UV absorption or conductivity
HETP calculation
Calculate the height equivalent of a theoretical plate (HETP) from the UV curve or
conductivity curve if sodium chloride is used as sample, as follows:
HETP = L/N
N=5.54 (V
e
/W
h
)
2
Variable Meaning
L Bed height
N Number of theoretical plates
V
e
Elution volume
W
h
Peak width at half peak height
V
e
and W
h
are in the same units
5 Operation
5
.3 Performance evaluation of the column
BPG Columns Operating Instructions 29193223 AB 73