Asymmetry calculation
C
alculate the asymmetry factor (A
S
) from the UV curve or conductivity curve if sodium
chloride is used as sample, as follows:
The peak should be symmetrical and the asymmetry factor as close to 1 as possible
(values between 0.8 and 1.8 are usually acceptable). A change in the shape of the peak
is usually the first indication of bed deterioration due to use.
The asymmetry factor A
s
describes the deviation from an ideal Gaussian peak shape
and is calculated from the peak width at 10% of peak height.
A
s
= b/a
5 Operation
5
.3 Performance evaluation of the column
74
BPG Columns Operating Instructions 29193223 AB
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