- 57 -
Images
Collage Image Border Padding (pixels) - Keeps an additional border of this many pixels around a
particle when cropping from the raw camera image. Changing this value has no effect on the calculated
properties of the particle. Typically this value should be set from 2-6 pixels.
Save Collage Images - Saves all images (segmented particles from a raw camera image) captured
during a run. This option is selected as a default setting.
Save Binary Collage Images - Saves a visual overlay of the pixels determined by VisualSpreadsheet
based on the user-defined Context Settings to be a particle. The binarization overlay is a useful
parameter to measure the efficiency of particle capture (see Close Holes above as well) and provide
feedback to the user as they adjust Context Settings.
Save Raw Camera Images - Saves each raw camera image (i.e. the image of the camera field of view).
The saved raw camera images can later be re-processed in File Processing Mode, for example to see the
effect of changing some of the context settings.
Max Raw Images - The maximum number of raw images to be saved when Save Raw Camera Images
is enabled. Due to the large size of each raw image, caution should be used when selecting a value for
this parameter to avoid reduced frame rates and excessive disk space usage.
Particles Defined By
Dark Pixels, Light Pixels, Both Dark and Light Pixels - These parameters and associated
Segmentation Threshold values provide the software with rules in regard to which pixels in the raw
image to consider as part of a particle. If a pixel within a raw image is darker/lighter/both than the same
pixel of the calibration image, and if the difference exceeds the user entered relative threshold value, it
will be considered part of a particle. Once all the pixels that comprise a particle are identified, the
software will continue to process the particle as instructed by the Context Settings.
Acceptable Region
Left, Right, Top, Bottom - The resolutions of the standard FlowCAM digital cameras are 1024 x 768 or
1280 x 960 pixels. Entering values into these fields that are less than the maximum range will
effectively constrain the field of view. Once constrained, only particles within and not touching these
constraints will be imaged and counted. Also see the Help > Advanced Tools > Advanced Settings…
section where the related topic of accepting full screen particles is defined.
To Next Page
Loading...
