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Context… Fluidics
Settings
Sample volume – A user-entered value for the amount of sample fluid that will be run through the flow
cell for the analysis. When using the syringe pump the software will automatically stop the run when the
specified sample volume has been analyzed (unless other Stop criteria are selected, see the Stop tab
description for more information).
If the settings entered for the experiment are outside the recommended operational specifications (i.e.
the combination of sample volume, flow rate, and auto image rate will result in more than 50% of the
sample volume to be used to perform the camera background calibration) an error message will appear.
If the volume needed to calculate the background calibration image exceeds the entered sample volume,
then the analysis will not be allowed to start.
Flow rate – A user-entered value for the rate of flow of sample through the flow cell during the
analysis. The flow rate is accurately controlled by the FlowCAM software when a syringe pump is
used. But when a peristaltic pump is used, it is up to the user to accurately set the flow rate as the
software has no direct control over a peristaltic pump.
AutoImage Rate – The number of frames per second that the camera will take during an AutoImage
mode analysis. The number of frames per second can be changed by selecting a new value from the
pull-down menu.
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