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Settings
Text and ruler color: The color of the Text and Ruler can be changed to a desired color. Click on the
Display color box. The color selection dialog box will appear. In this dialog box, select the desired
color. Use the same method to change the other color boxes in this window.
Particle box color: Allows the user to change the color of the segmentation boxes.
Axis grid color: Allows the user to change the color of the axis grid.
Axis ticks every [user set value] µm: Allows the user to change the spacing of the axis ticks.
Show acceptable region: Displays a colored border indicating the area set for the acceptable region.
The acceptable region border is only visible when the acceptable region is smaller than the full camera
capture area.
Show elapsed time: Displays the amount of time that the camera has been capturing images.
Show time remaining: Displays the approximate amount of time that remains before the analysis
automatically terminates.
Show particle count: Displays the total number of particles that have been counted.
Show pixel intensity extremes: Displays the Mean, Min, and Max Intensity values as camera images
are being captured. Note that the Intensity values range from 0 to 255.
Show fluorescence/scatter: Displays the fluorescence and/or scatter value for FlowCAM models
configured for Trigger Mode. When enabled, this parameter will not be displayed on the Setup and
Focus window until a Trigger Mode analysis is initiated.
Show percentage of used images. Displays the percentage of used images (an image is used if at least
one particle was segmented and captured from it).
Show particles per used image. This value indicates the average number of particles that are present
and segmented/captured from “used” raw camera images, i.e., from images in which at least one particle
was segmented and captured. In Trigger Mode, this parameter is valuable to help the user determine if
the sample solution is sufficiently dilute to produce optimal Trigger Mode results. In Trigger Mode, this
value should be as close as possible to 1.00 (with a maximum value of 1.10 for optimal triggering). If
this value is exceeding 1.10 in Trigger Mode, the sample may need to be diluted.
Show frame rate: Displays the actual frame rate value (which may be different than the value set in the
camera dialog window).
Show fluid volume imaged: Displays the fluid volume actually imaged by the FlowCAM.
Show sample volume processed: Displays the fluid volume processed by the FlowCAM (available
only if a syringe pump is used).
Show flow rate: Displays the fluid flow rate (available only if a syringe pump is used).
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